Journal: Journal of affective disorders

Article Title: Imipramine induces hyperactivity in rats pretreated with ouabain: Implications to the mania switch induced by antidepressants

doi: 10.1016/j.jad.2021.12.021

Figure Lengend Snippet: Effects of the artificial cerebrospinal fluid (aCSF) or ouabain (OUA, a Na+/K+-ATPase inhibitor) intracerebroventricular infusion on the 4-hydroxy-2-nonenal (4-HNE, a, b) and carbonyl (c, d) contents, as well as on the glutathione peroxidase (GPx, e, f) and glutathione reductase (GR, g, h) activities in rat frontal cortex and hippocampus, 14 days after the recovery period following the stereotaxic surgery (n = 7, per experimental group). Effects of the intraperitoneal administration of the antidepressant drug imipramine (IMI) or saline (Sal, NaCl 0.9%) were also evaluated in this context, aiming to develop an animal model of mania switch. Data were analysed by two-way analysis of variance followed by Tukey’s test when F was significant. Values are expressed as μg/mL protein (4-HNE), nmol/mL (carbonyl) or nmol min−1 mL−1 (GPx and GR) (mean ± standard error of mean). *p < 0.05, as compared to aCSF + Sal group. #p < 0.05, as compared to OUA + Sal group.

Article Snippet: Oxidative stress biomarkers The oxidative lipid damage in the cerebral structures was estimated through the 4-hydroxy-2-nonenal (4-HNE) content, using the assay kit provided by Cell Biolabs (Cell Biolabs, Inc., San Diego, California, USA; catalog no. STA-338).

Techniques: Saline, Animal Model

Journal: International Journal of Biological Sciences

Article Title: Neuraminidase 1 deficiency attenuates cardiac dysfunction, oxidative stress, fibrosis, inflammatory via AMPK-SIRT3 pathway in diabetic cardiomyopathy mice

doi: 10.7150/ijbs.65938

Figure Lengend Snippet: shNEU1 attenuated diabetes-induced oxidative stress in the hearts. A-B. Western blot and quantitative analysis showing the protein levels of p67 phox and SOD2 in vehicle and shNEU1 treated mice (n =6). C. Endogenous antioxidants (GSH) content (n=6). D. Total SOD activity in diabetic mice (n=6). E. Lipid peroxidation in diabetic hearts (n=6). F. 4-HNE (n=6). G. NADPH oxidase subunits mRNA expression by real time RT-PCR (n=6). * P <0.05 vs. Saline+Vehicle group; # P <0.05 vs. STZ+Vehicle group.

Article Snippet: To further assess oxidative stress level, myocardial malondialdehyde (MDA) or 4 hydroxynonenal levels (4-HNE) levels, SOD activities and glutathione (GSH) oxidized glutathione (GSSG) levels in the myocardial tissues were determined using commercially available kits procured from Beyotime Co. (Nantong, China) according to previous study .

Techniques: Western Blot, Activity Assay, Expressing, Quantitative RT-PCR, Saline

Journal: BMC Cancer

Article Title: Acyclic retinoid and angiotensin-II receptor blocker exert a combined protective effect against diethylnitrosamine-induced hepatocarcinogenesis in diabetic OLETF rats

doi: 10.1186/s12885-018-5099-6

Figure Lengend Snippet: Effects of acyclic retinoid (ACR) and angiotensin-II receptor blocker (ARB) on liver steatosis, oxidative stress and inflammation. a - c Hepatic levels of triglyceride (TG) ( a ), 4-Hydroxynonenal (4-HNE) ( b ) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) ( c ) in experimental rats. d - h Relative mRNA expression levels of rat Tnfa ( d ), Il6 ( e ), Il1b ( f ), Ccl2 ( g ) and Serpine1 ( h ) in the liver of experimental rats. LETO; Long-Evans Tokushima Otsuka rat, OLETF; Otsuka Long-Evans Tokushima Fatty rat. 4-HNE and 8-OHdG levels are indicated as ratio to LETO control group ( b and c ). Relative mRNA expression levels were measured by quantitative RT–PCR (qRT–PCR), and GAPDH was used as internal control for qRT–PCR ( d - h ). Data are mean ± SD ( n = 10). *, P ≤ 0.01 between each group

Article Snippet: The hepatic 4-Hydroxynonenal (4-HNE) content was measured using the 4 Hydroxynonenal ELISA Kit (MyBioSource, San Diego, CA, USA) as per the manufacturer’s protocol.

Techniques: Expressing, Control, Quantitative RT-PCR